rabbit antibodies against β actin  (Proteintech)

Journal: Scientific Reports

Article Title: Comparative study of palmitoleic acid, sea buckthorn oil, and lovastatin in hepatocellular steatosis model

doi: 10.1038/s41598-026-37006-y

Figure Lengend Snippet: Effects of cPOA, tPOA, SBO, and digested SBO on intracellular cholesterol content (A, B) and HMGCR gene expression (C, D) in control (A, C) and FFA-induced steatotic (B, D) HepG2 cells. Cells were exposed to indicated concentrations of compounds or 5 µM lovastatin. Results represent at least 3 independent biological samples with 3 repeats each ( n = 3). The bars represent the means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 versus control. β ACT , β-aktyna; cPOA, cis -palmitoleic acid; FFA, free fatty acid; OA/PA, oleic acid/palmitic acid; SBO, sea buckthorn oil; tPOA, trans -palmitoleic acid.

Article Snippet: Antibody against β-actin (8457, 1:10,000 dilution) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Gene Expression, Control

Journal: Scientific Reports

Article Title: Comparative study of palmitoleic acid, sea buckthorn oil, and lovastatin in hepatocellular steatosis model

doi: 10.1038/s41598-026-37006-y

Figure Lengend Snippet: Effect of cPOA, tPOA, SBO, and SBO digested on Rap1A prenylation in HepG2 cells. Control (A, B and E, F) and FFA-induced steatotic cells (C, D and G, H) were exposed to indicated concentrations of compounds or 5 µM lovastatin (Lov) for 48 h. Subsequently, cells were lysed and isolation of cytosolic and membrane proteins was performed. Cytosolic fractions containing unprenylated proteins were western blotted for Rap1A and β-actin (A, C and E, G), and the immunoblot bands were quantified by densitometry analysis, normalized to β-actin, and presented as a percentage of controls (B, D and F, H). Lovastatin was used as a positive control. Treatment of cells with lovastatin should prevent the Rap1A accumulation at the membrane. Data represented mean ± SEM from at least three independent experiments ( n = 3); * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001. Cropped gels are presented here; full-length gels and blots without cropping are included in the Supplementary Information file. cPOA, cis -palmitoleic acid; FFA, free fatty acid; OA/PA, oleic acid/palmitic acid; SBO, sea buckthorn oil; tPOA, trans -palmitoleic acid.

Article Snippet: Antibody against β-actin (8457, 1:10,000 dilution) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Control, Isolation, Membrane, Western Blot, Positive Control